Welcome to MeLiS

Mechanisms in Integrated Life Sciences

Université Claude Bernard Lyon 1 - CNRS UMR 5284 - INSERM U1314

MeLiS teams are interested in the molecular mechanisms operating in a variety of cellular compartments, from cilia, membranes, axons, to synapses and circuits, with a focus on neuronal and muscular cells.
We investigate a range of biological processes and their alterations in human diseases affecting developing, adult and aging organisms.
We use a diversified approach taking advantage of a large panel of models, including: drosophila, C. elegans, zebrafish, chicken, mouse, and human. Our studies combine genetics, large-scale molecular analyses, in vivo manipulations, behavioral assays, with strong emphasis on microscopy techniques (super-resolution microscopy, expansion microscopy, in vivo 2-photon, light sheet microscopy, electron microscopy) and image quantification (segmentation, animal tracking, time-series).

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MeLiS — Breaking News

SUNRISE - Rise of the Structure-encoded UNC5 interactome in aggressive behaviours of Neuro-developmental tumours

Potentialisation des fonctions physiologiques de l’interactome UNC5 et comportements agressifs des tumeurs neurodéveloppementales

2024-11-07

Valérie Castellani, Elena Seiradake (Université d‘Oxford) et Daniel Del Toro (Université de Barcelone) sont lauréats de l'appel d'offer ERC Synergie Grant 2024.

Extrait du communiqué de presse du CNRS

"Comprendre les singularités des tumeurs pédiatriques, souvent initiées dès le stade embryonnaire et bien distinctes des cancers de l’adulte est un défi majeur. Étonnamment, les cellules malignes ressemblent à leurs cellules d’origine et, malgré un faible taux de mutations, peuvent se révéler hautement agressives et résister aux traitements. Ces propriétés pourraient-elles être liées au contexte particulier de leur émergence ? Le projet SUNRISE, centré sur les tumeurs neurodéveloppementales et principalement le neuroblastome, s’intéresse à cette piste. [...] Nous espérons révéler des mécanismes uniques aux cancers pédiatriques en lien avec leurs cellules d’origine et ouvrir la voie à des thérapies ciblées innovantes."

MeLiS — Publication highlights

Time-series reconstruction of the molecular architecture of human centriole assembly

Laporte MH, Gambarotto D, Bertiaux É, Bournonville L, Louvel V, Nunes JM, Borgers S, Hamel V, Guichard P.

This work by Marine Laporte (presently in Team Durand) in Cell in April 2024, reveals centriolar assembly dynamics through a time-series reconstruction of high-resolution images. Using expansion microscopy, we captured snapshots of assembling centrioles and precisely mapped the appearance, disappearance, positioning, and behavior of 24 centriolar proteins. This enabled the reconstruction of a pseudotime series of centriole assembly and the determination of each protein's growth rate.
This strategy, which we believe will serve as the gold standard for centriole assembly studies, will be applied to our current project aimed at investigating the regulation of ciliary membrane dynamics.